Single-nuclei RNA-seq for CADASIL understanding and therapeutic target discovery
Awardee: Israel Fernández Cadenas
Institution: Fundació Privada Institut de Recerca de l’Hospital de la Santa Creu i Sant Pau
Grant Amount: $60,228
Funding Period: February 1, 2022 - January 31, 2023
Summary:
The aim of this project is to understand the reason why CADASIL is produced, and possible factors associated with the severity of the disease. To do this, we will use a new and innovative strategy with omic technology (single-nuclei RNA-seq analysis) to obtain transcripts and pathways associated with the disease and its severity. Based on this information, we purpose to find therapeutic targets overexpressing/inhibiting the molecules found to be significant in the single-nuclei RNA-seq study and those found significant in other omic studies of CADASIL already published, to evaluate later the benefits in our human cellular model (pattern of aggregation of Notch3).