Deep phenotyping of mouse models of UBE3A gain-of-function

Awardee: Jason Yi

Institution: Washington University School of Medicine

Grant Amount: $47,158.00

Funding Period: February 1, 2024 - January 31, 2025


Summary:

Dup15q syndrome is caused by a duplication or triplication of maternal chromosome 15q11-13 whereas individuals with paternal duplications are typically developing. There are more than 20 genes within chromosome 15q11-13, but among them, Ube3a is the only gene expressed exclusively from the maternal allele in neurons. These observations strongly suggest that excessive UBE3A protein activity is the major driver of disease phenotypes in Dup15q syndrome. This proposal will perform deep phenotypic analysis of an allelic series of mice that possess gain-of-function mutations in Ube3a of increasing severity. By doing so, our study will identify specific phenotypes in mice that are caused by excessive UBE3A protein activity. These studies will provide valuable models and information that can be leveraged to design therapeutic strategies for this disorder.

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Preclinical Validation of potential GLUT1 inducing agents for GLUT1 Deficiency Syndrome (GLUT1 DS).

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Development of a Novel Viral Vector Gene Therapy Approach to treat CACNA1A Cerebellar Disorders